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KMID : 0358419940370071431
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Korean Journal of Obstetrics and Gynecology
1994 Volume.37 No. 7 p.1431 ~ p.1439
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Determination of the Optimum Dosages of 8-Methoxypsoralen and Ultraviolet A Light for the Photochemotherapy
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한구택
유기성/최명희/곽은정/남궁성은/김수평/김승조/이헌영
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Abstract
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Photochemotherapy using the photosensitizer and ultraviolet A light is a relatively new and potentially selective experimental approach to the treatment of malignant neo-Plasms. The combination of 8-methoxypsoralen plus ultraviolet A
light(320~400
nm)
is used in the treatment of a hyperproliferative disease of the skin, due to its antiproliferative and cytotoxic effects associated with the DNA photoaddition of 8-methoxypsoralen.
In this study, DNA interstranded cross-links were induced in Hind III-linearized plasmid DNA after in vitro treatment with various concentrations of 8-methoxypsoralen (0.0005~15.0μg) and ultraviolet A light to determine the usable doses of
8-methoxypsoralen and irradiation doses of ultraviolet A light for its future use in gynecologic neoplasms. The double stranded-DNA formed by 8-methoxypsoralen photoinduced cross-links were appeared from the 0.125μg of 8-methoxypsoralen using
20KJ/m*
of ultraviolet A light dose, showing increased proportion of double stranded-DNA with the increasing doses and vice versa.
These results suggested that the dosages over 0.125μg of 8-methoxypsoralen will be able to use. In the treatment for some gynecologic neoplasms under the irradiation of 20 KJ/m* of ultraviolet A light.
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